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Image Search Results
Journal: Scientific Reports
Article Title: β-nicotinamide mononucleotide (NMN) production in Escherichia coli
doi: 10.1038/s41598-018-30792-0
Figure Lengend Snippet: The growth curve (OD600) of transformed Escherichia coli BL21(DE3)pLysS with pET-28a(+) vector carrying nadV (NAMPT) gene ( A – C ) respectively untransformed culture ( D ) in LB medium supplemented with 0.1%, 1% and 5% NAM.
Article Snippet: The DNA sequences of nadV genes of these two organisms obtained by synthesis (GenScript) were cloned in pET-28a+ vector while NAMPT from Mus musculus was conveniently available on pET-28a(
Techniques: Transformation Assay, Plasmid Preparation